ml si3 Search Results


ml si3  (MedChemExpress)
94
MedChemExpress ml si3
Ml Si3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ml-si3  (TargetMol)
99
TargetMol ml-si3
Ml Si3, supplied by TargetMol, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1r  (MedChemExpress)
94
MedChemExpress 1r
Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor <t>(1R,2R)-ML-SI3</t> (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.
1r, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
1r - by Bioz Stars, 2026-03
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ml-si3  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH ml-si3
Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor <t>(1R,2R)-ML-SI3</t> (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.
Ml Si3, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ml-si3 - by Bioz Stars, 2026-03
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ml-si3  (MedKoo Inc)
90
MedKoo Inc ml-si3
Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor <t>(1R,2R)-ML-SI3</t> (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.
Ml Si3, supplied by MedKoo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ml-si3/product/MedKoo Inc
Average 90 stars, based on 1 article reviews
ml-si3 - by Bioz Stars, 2026-03
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atomic model of ml-si3-bound trpml1  (Databank Inc)
90
Databank Inc atomic model of ml-si3-bound trpml1
Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor <t>(1R,2R)-ML-SI3</t> (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.
Atomic Model Of Ml Si3 Bound Trpml1, supplied by Databank Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
atomic model of ml-si3-bound trpml1 - by Bioz Stars, 2026-03
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si 3 ml−1  (BIOTAGE)
90
BIOTAGE si 3 ml−1
Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor <t>(1R,2R)-ML-SI3</t> (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.
Si 3 Ml−1, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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si 3 ml−1 - by Bioz Stars, 2026-03
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Image Search Results


Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor (1R,2R)-ML-SI3 (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.

Journal: Scientific Reports

Article Title: Obinutuzumab induces lysosomal destabilization via sphingomyelin-dependent inhibition of TRPML2

doi: 10.1038/s41598-026-38087-5

Figure Lengend Snippet: Lysosomal stress and TRPML inhibition enhance obinutuzumab-mediated direct cell death. ( a ) LMP and DCD were measured in Raji cells treated with hypertonic medium (200 mM sucrose) or hypotonic medium (60% distilled water in complete culture medium) for 2 h in the presence of OBI (Hereafter, the concentration of OBI is 10 μg/ml unless otherwise stated). LMP and DCD were evaluated by LysoTracker Green and propidium iodide (PI) staining. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( b ) OBI-induced LMP and DCD were assessed in Raji cells co-incubated with increasing concentrations of the TRPML inhibitor (1R,2R)-ML-SI3 (10 µM). In parallel, cells pretreated overnight with the PIKfyve inhibitor Apilimod (50 nM) showed enhanced OBI responses, but this effect was abolished when TRPMLs were simultaneously inhibited. One-way ANOVA with Dunnett’s multiple-comparisons test (vs. control). ( c ) Dose-dependent enhancement of OBI-induced DCD was observed following 2-h co-incubation with a fixed concentration of the (1R,2R)-ML-SI3 (10 µM, over 2 h incubation, this (1R,2R)-ML-SI3 treatment condition was used unless otherwise stated). ( d , e ) Time-dependent enhancement of OBI-induced LMP ( d ) and DCD ( e ) were assessed in TRPMLs-targeting siRNA cell lines. ( f ) Lysosome enlargement and fluorescence intensity were indicated by LT Deep Red staining, then imaged by confocal microscopy. (1R, 2R)-ML-SI3, hypertonic, and hypotonic medium were incubated 2 h. Scale bars in all images represent 5 µM. ( g , h ) Quantification of cathepsin B release induced by OBI and (1R,2R)-ML-SI3 treatment. ( g ) Raji cells were treated with (1R,2R)-ML-SI3, hypertonic medium (200 mM sucrose), or hypotonic medium (60% distilled water in complete medium) for 2 h, and with OBI for 4 h. Cathepsin B releases were visualized by confocal microscopy by immunostaining. ( h ) The summary of the release data shows significant cathepsin B secretion in response to OBI treatment, which was further modulated by (1R,2R)-ML-SI3 and osmotic stress conditions. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001 compared to obi-treated group.

Article Snippet: For drug treatments, (1R,2R)-ML-SI3 (HY-134819A; MCE), ML2-SA1 (axon Medchem), and 3- O -methyl Estradiol (#42995, Cayman Chemical) were administered for 2 h at 37 °C, followed by OBI (0.3 μg/ml) treatment for an additional 2 h. Endocytosis inhibition was performed using filipin (HY-N6716, MCE), dynasore (S8047, Sellekchem), pitstop2 (HY-115605, MCE), Sphingomyelinase (S7651-10UN, Sigma), or heat-inactivated Sphingomyelinase for 30 min at 37 °C before antibodies treatment (10 μg/ml, IgG, RTX, OBI).

Techniques: Inhibition, Concentration Assay, Staining, Control, Incubation, Fluorescence, Confocal Microscopy, Immunostaining